Poster Presentation 50th Lorne Proteins Conference 2025

Investigating complex molecular interactions using novel SPR analysis approaches   (#216)

Emma Dal Maso 1 , Alexander Fish 2 , Elin Sivertsson 3
  1. Cytiva , Mount Waverley, VIC, Australia
  2. Netherlands Cancer Institute, Amsterdam, Netherlands
  3. Cytiva , Uppsala, Sweden

The DNA mismatch repair system was studied with Dual, ABA and Poly injection commands. The results show the importance of binding order and dose dependencies. The Poly command was used to study interactions of the receptor binding domain (RBD) of spike glycoprotein SARS-CoV-2 with ACE2 receptor, an antibody against RBD and mouse anti-human antibody. Applying referencing with varying injection sequences of components and blanks reveals convoluted aspects of the interactions.